Monday, May 20, 2013 Online Medical Microbiology guide

Dear all
I have recently launched a new website microbeonline. Which i am trying to make a complete online medical microbiology guide.
I felt the need of site, because it gives more flexibility for me in content management part and also will be helpful to visitors to find the content of their interest and to navigate to different posts.
I also request you to join facebook page of microbeonline
I will be waiting for your feedback so that i can post useful and relevant content. Let me know the improvements needed in microbeonline

Thursday, April 18, 2013

Pathogenicity islands (PAIs) of Human Pathogen: Properties and Types

 Pathogenicity islands (PAIs) are large group of mobile genetic elements that are associated with pathogenicity and are located on the bacterial chromosome. The concept of PAI was founded in the late 1980s by Jörg Hacker and colleagues.

Figure: Genetic Structure of PAI

SOP for processing of urine specimen for laboratory diagnosis of Extended spectrum Beta lactamase producing E.coli

 Collection of specimen:
  1. Instruct the patient to collect midstream urine.
  2.  Give the patient a sterile, dry, wide necked, leak-proof container and explain the importance of collecting a specimen with as little contamination as possible.
  3. Instruct the female patients to cleanse the area around the urethral opening with clean water, dry the area, and collect the urine with the labia held apart.
  4. Collect about 10-15 ml of urine. If the patient is in renal failure or a young child, it may not be possible to obtain more than a few milliliters of urine.

Labelling and rejection criteria:
Label the specimen container appropriately with registration/collection number, patient name, type of specimen, date and time of collection and the tests requested.

  1. As soon as possible, deliver the specimen with a request form to the laboratory.
  2. Keep the urine in refrigerator at 4ºC, if immediate delivery to the laboratory is not possible.
  3. Add boric acid (0.1g/10ml) to the urine, if a delay in delivery of more than 1hr is anticipated.

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