Name of the organism
|
Sample
|
Microscopy
|
Blood examination
|
Direct examination
|
Other
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Ascarisis lumbricoides
|
Stool or vomit.
Bile by duodenal intubation.
|
- Egg: direct microscopical examination of a saline emulsion of the stool.
- Concentration: flotation method
- Unfertilized eggs don’t float in salt solution.
- Solitary male harbouring: no egg.
- Egg in bile.
|
Eosinophilia during early stage of invasion. If present in the intestinal phase suggests associated strongyloidiasis and toxocariasis.
|
Finding of adult worms. Antihelminthic drug increase expulsion of worms.
|
X-ray with barium emulsion.
Allergic dermal rxn(scratch test) with powdered Ascaris antigen.
|
Giardiasis
|
Freshly passed stool. Bile (duodenal intubation)
|
Demonstration of Giardia trophozoite (in diarrhoeic stool) and cyst.
| |||
Intestinal amoebiasis
|
Stool
Blood (blood shows moderate lecocytosis)
|
Cellular exudates: scanty, containing only nuclear mass (Pyknotic bodies) of only a few pus cells, macrophages and epithelial cells.
Clumped RBC:reddish yellow or yellow green in color
Charcot-Lyden crystals.
*not pathognomonic.
*Suggests careful examination of stool.
*Diamond shaped or whetstone shaped crystals, clear and refractile (5-50µm in size).
Demonstration of E.histolytica by examining trophozoites in acute cases, characteristic movement & presence of ingested RBC.
Moderate leucocytosis.
|
An offensive dark brown, semifluid stool, acid in rxn, admixed with blood, mucus and presence of much fecal matter.
|
Serology always negative. Although there is tissue invasion, it has not existed long enough to produce detectable antibody.
| |
Amoebic live abscess
|
Pus by exploratory puncture.
Liver biopsy in miliary amoebic hepatic abscess.
Stool.
|
Demonstration of trophic form of E. histolytica.
Present in less than 15% cases of amoebic liver abscess.
|
Leukocytosis (15,000-30,000 per mm3) of blood.
DC shows Neutrophil granulocytes to be 70-75%.
|
OTHERS: Intradermal test: erythema.
Radiological Examination: shows evidence of basal pleurisy.
Hepatic Photoscan.
|
Presence of specific antibodies circulating in the blood of infected individual.
CFT, precipitin test, immobilization test, Test of Goldman, IHA, Passive cutaneous anaphylaxis.
|
Pulmonary amoebiasis
|
Sputum
|
Demonstration of trophozite of E. histolytica.
Expectorated pus when examine fresh, may demonstrate the motile form(trophozoite) of E.histolytica.
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This blog contains Multiple Choice questions in Microbiology and related disciplines (Virology, Parasitology, Mycology, Bacteriology, Genetics) and Notes for all the related disciplines.
Tuesday, April 20, 2010
Parasitology Note
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