Tuesday, February 28, 2012

Syllabus of Medical Microbiology: Virology, Parasitology and Mycology of M.Sc 2nd year Tribhuvan University


Course Title: Virology, Mycology, Parasitology Full Marks: 100
Course No: MB 622 Pass Marks: 40
Nature of Course: Theory

Course Objectives:Up on completion of the course the students will
a.
be able to understand systematics of medically important viruses, fungi and parasites
b.
be able to understand principles, procedures and applications of diagnostic methods useful to detect infections causes by viruses, parasites and fungi.


Virology-60 Hrs.
History and principles of virology, Taxonomy and replication strategies of various viruses and Bacteriophages. Classification, structure, medical importance, pathogenesis and laboratory diagnosis of Poxviruses, Herpes viruses, Adenoviruses, Picornavirus, Orthomyxovirus, Paramyxovirus, Arbovirus, Rhabdo viruses, Hepatitis virus, Retrovirus (HIV, HTLV etc), emerging viruses (SARS, Oncogenic virus), prions, Antiviral drugs, Principles of bio-safety and requirements of virology laboratory. Technique of Diagnostic virology: Cultivation and purification of viruses, Principle and application of serodiagnostic methods-hemagglutination and haemagglutination inhibition tests, Complement fixation, neutralization, Western blot, RIPA, Flowcytometry and Immunohistochemistry. Nucleic acid based diagnosis- Nucleic acid hybridization, polymerase chain reaction, microarray and nucleotide, sequencing. Microscopic techniques- Fluorescence, confocal and electron microscopic techniques.

Mycology: 30 Hrs
Classification, general structure, physiology, pathogenesis and medical importance of fungi; Aspergillus, candida, fusarium, Cryptococcus, Histoplasma, Trichophyton, Epidermophyton, Blastomyces, Cryptococcus, Tinea, Microsporum, Coccidioidomyces, Paracoccidioidomyces, Laboratory diagnosis of fungal disease: Subcutaneous mycosis, Systemic Mycoses, Opportunistic Mycoses, Fungal toxin and Allergies, Antifungal drugs and Drug susceptibility. Different method for detection of toxins.


Parasitology: 30 Hrs
Overview of parasite, host, parasitism, parasitic diseases prevalent in Nepal and world, Classification, geographical distribution, habitat, morphology, life cycle, pathogenicity (mode of infection, pathogenesis and pathology), laboratory diagnosis and prevention and control of medically important protozoa and helminthes: Entamoeba histolytica, Giardia lamblia, Trichomonas, Ascaris, Anacyclostoma and Necator, Enterobius vermicularis, Trichuris trichiura, Strongloides, Taenia, Echinococcus, Hymenolepsis nana, Brugia, Loaloa, Onchocerca, Dracunculosus, Plasmodium, Leishmania, Toxoplasma, Wucheria.

Recommended Books
  1. Topley, W.W.C. (Author), Wilson, G.S. (Author), Parker, M.T. (Editor), Collier, L.H. (Editor) (1990) Topley and Wilson's Principles of Bacteriology, Virology and Immunity, Hodder Arnold.
  2. Baron, E.J. Peterson, L.R and finegold, S.M. (1990) Bailey and Scott's Diagnostic Microbiology, Mosby.
  3. Stephenson J.R. (Editor), Warnes A. (1998), Diagnostic virology Protocols: Methods in Molecular Medicine, Humana Press.
  4. Fields B.N. Knipe D.M., Howley P.M, Chanock R.M. Melnick J. L, Monath T. P. Roizman B., and straus S.E. (2001), Fields Virology Vol 1 and 2. 3rd edition. Lippincott-Raven, Philadelphia PA.
  5. Flint, S.J., Enquist, L.W., Krung R., Racaniellos V.R, and Skalka, A.M. (2000). Principles of virology, Molecular Biology, Pathogenesis and Control. ASM Press
  6. Larone D.H. (2002). Medically important fungi: a guide to identification. ASM Press
  7. Chartejee K.D. (Editor) (1981) Parasitology (Protozoology and Helminthology), 12th edition, Chaterjee Medical Publishers, India.

2 comments:

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  2. Does anyone have syllabus for the following courses (annual system) of M.Sc and B.Sc Microbiology of Tribhuvan University?

    M.Sc 2nd – Human Anatomy & Physiology
    B.Sc 1st – Zoology
    B.Sc 2nd – Zoology
    B.Sc 3rd – Research Methodology Bio-statistics

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