Syllabus of Medical Microbiology Practical of M.Sc 2nd year Tribhuvan University

Course Title: Medical Microbiology Full Marks: 100
Course No: MB 624 Pass Marks: 40
Nature of Course: Practical Year: II

Course Objectives
up on completion of the course the students will

1. have advanced skills on processing clinical specimens for microbiological analysis
2. have advanced skills on isolation, characterization, identification, and typing of medically important bacteria, virus, fungi and parasites.
   

   Course Contents
   
   
   
   Bacteriology

   1. Isolation and identification of medically important facultative anaerobic, microaerophilic and anaerobic bacteria by conventional methods.
   2. Mycobacterium microscopy and culture
   3. Serotyping, bacteriophage typing and molecular typing of medically important bacteria.
   4. Antibiotic susceptibility testing of facultative anaerobic, microaerophilic and anaerobic bacteria and Mycobacterium
   5. Serological/Immunological tests for detection of bacterial infections-Test Kits: RPR, TPHA, ASO titre, Widal test etc. Direct/Indirect immunofluorescence test, precipitation test, Mantoux test, ELISA, Western blot, CFT, RIA.
   6. Collection and Processing and culture of Stool, Sputum, Blood, Urine, Throat/Nasal swab, Vaginal swabs, Pus and identification of etiological agent.
   7. Laboratory diagnosis of Enteric fever
   8. Laboratory diagnosis of of Cholera
   9. Laboratory diagnosis of Meningitis
   10. Microscopy of Treponema
   11. Laboratory diagnosis of eye infections.
   12. Laboratory diagnosis of Ear infections.
   13. Laboratory diagnosis of oral infections.
   14. Indoor and outdoor air quality monitoring of hospital environment.
   15. Drinking water quality analysis.

   
   

Virology, Mycology and Parasitology

1. Preparation of media and reagents, sterility checking, Glassware decontamination, washing, sterilization.
2. Propagation of viruses; Tissue culture and egg inoculation technique for the isolation of common medically important viruses;Routes of inoculations in embryonated eggs.
3. Preparation of virus stocks; plaque assay and determination of TCID50
4. Detection of Virus Antigen by ELISA; Immunofluorescence assay; Heamagglutination; Agar gel diffusion; Polymerase Chain Reaction
5. Electron Microscopy
6. Isolation,, Enumeration, Plaque assay, Preparation of high titre of bacteriophage stocks and One step growth curve experiments.
7. Preparation of fungal stains and staining techniques
8. Fungal culture techniques
9. Isolation and Characterization of medically important fungi from clinical specimens
10. Isolation and characterization of dimorphic fungi
11. Microscopic observation of medically important protozoans and helminthic parasites-Entamoeba, Giardia, Plasmodium, Leishmania, Taenia, Ascaris etc.
12. Stool sample collection and processing for observation of parasites by microscopy
13. Occult blood test in the stool sample.
14. Stool culture
15. Laboratory diagnosis of blood and tissue parasites-preparation of thick and thin smear of blood sample, staining and detection of blood and tissue parasites.
16. Laboratory diagnosis of vector borne diseases and identification of common insect vectors.Human Anatomy and Physiology

1. Anatomical observation of human skeleton and organ systems
2. Microscopic observation of histological and anatomical slides
3. Experiments on Human Physiology.