Course Syllabus: TU M.Sc Microbiology First year:Practical Course on (MB511+MB 512)

Practical I

Course Title: Practical Course on (MB511+MB 512) Full Marks: 50

Course No: MB516 Pass Marks: 20

Nature of course: Practical Year: I

Objectives

Upon the completion of the course, students will have

• practical skills on handling, isolation, identification and enumeration of bacteria, yeast and molds.
• advanced practical skills on molecular techniques to study microbial genetics.
• advanced practical skills on immunological and serological techniques.

I. Microbial Structure, Physiology and Genetics

Bacterial Morphology

• Microscopy and Staining

Microbial Physiology

• Measurement of growth of bacteria
• Enumeration techniques
• Biomass determination
• Determination of bacterial growth curve in broth medium
• Effect of environmental factors on bacterial growth
• Temperature, pH, UV radiation, nutrients, water activity, gaseous requirements
• Degradation experiments
• Cellulose, Starch, Gelatin, Casein, Tween 80
• To study inhibitory effect of lysozyme and detergents on bacterial cell wall
• Conventional biochemical testing for identification of enterobacteriaceae family
• Biochemical identification of unknown bacteria (Gram positive, gram negative)
• Antimicrobial Susceptibility Test

Course Syllabus: TU M.Sc Microbiology First year:Practical Course on (MB 513+MB 514)

Practical II

Course Title: Practical Course on (MB 513+MB 514) Full Marks: 50

Course No. MB 517 PassMarks: 20

Nature of Course: Practical Year: I

Objectives

Upon the completion of the course, students will have

• practical skills on various biochemical techniques and instrumentation, required for conducting analysis, research.
• practical skills on production, purification and quality control of industrial products of microbial origin.
• practical skills on production, purification, evaluation and sterility testing of pharmaceutical products.

Course Syllabus: TU M.Sc Microbiology First year:Epidemiology, Research Methods and Biostatistics

Epidemiology, Research Methods and Biostatistics

Title: Epidemiology, Research Methods and Biostatistics Full marks: 50

Course No.: MB 515 Pass marks: 20

Nature of the course: Theory Year: I

Objectives

Upon the completion of the course students will have knowledge on

• Scope and application of Epidemiology
• Research methods in Microbiology
• Application of Biostatistical methods in microbiological research.

Epidemiology

Overview of Epidemiology

Scope and applications of Epidemiology, Relation of Epidemiology with Microbiology 2 hrs

Course Syllabus: TU M.Sc Microbiology First year: Microbial Structure, Physiology and Genetics

Microbial Structure, Physiology and Genetics

Course Title: Microbial Structure, Physiology and Genetics Full Marks: 100

Course No. MB 511 Pass Marks: 40

Nature of Course: Theory Year: I

Objectives

Upon the completion of the course students will have advanced knowledge on

• Bacterial and viral taxonomy
• The microbial genetics and application.
• Structure and physiology of bacteria, virus and fungi.
• Growth and recovery of bacteria and bacteriophage.

Microbial Structure and Physiology

Bacterial Classification

History, Fundamental and new approaches to bacterial taxonomy and nomenclature, Bacterial phylogeny, Characteristic of major families of bacteria (Gram positive, Gram negative, Mycobacteria, Actinomycetes, Rickettsia, Chlamydia, Mycoplama) 14 hrs

Course Syllabus: TU M.Sc Microbiology First year:Microbial Biotechnology and Pharmaceutical Microbiology

Microbial Biotechnology and Pharmaceutical Microbiology

Course Title: Microbial Biotechnology and Pharmaceutical Microbiology Full Marks: 100

Course No.: MB 514 Pass Marks: 40

Nature of course: Theory Year: I

Objectives

Upon the completion of the course students will have advanced knowledge on

• Scope and application of Microbial Biotechnology
• Production, purification, and product recovery of industrial products of microbial origin
• Applications of Pharmaceutical Microbiology and quality control systems applied in pharmaceutical industry

Microbial Biotechnology

Overview: History, Scope, Areas, Application, Risk and Hazards of Biotechnology;

Gene Transfer Techniques: Vector Gene transfer (based on Ti and Ri Plasmid, intermediate and helper plasmid, binary vector, virus as vector), Gene transfer techniques using Agrobacterium; Animal Cell culture Technology, Plant cell and Tissue Culture Technology 10 hrs

Course Syllabus: TU M.Sc Microbiology First year:Biochemistry and Instrumentation

Biochemistry and Instrumentation

Course Title: Biochemistry and Instrumentation Full Marks: 100

Course No: MB 513                                                        Pass Marks: 40

Nature of Course: Theory Year: I

Objectives

Upon completion of the course, students will have

• Advanced knowledge on general and microbial biochemistry.
• Advanced knowledge on principles and procedures of various biochemical techniques and instrumentation required for conducting analysis and research.

Overview of Principles of Biochemistry, Bio-molecules 2 hrs

Carbohydrates

Classification, Structures and Biological functions of Carbohydrates and Glycoproteins 6 hrs

Course Syllabus: TU M.Sc Microbiology First year:Immunology

Immunology

Course Title: Immunology Full Marks: 100

Course No.: MB 512                                                                                   Pass Marks: 40

Nature of Course: Theory Year: I

Objectives

Upon completion of the course, students will be able to

• Understand basic immunology
• Understand current immunological techniques and assays
• Be familiar with immunopathology of viral, bacterial, parasite, autoimmune, tumor and fungal diseases.
• Be able to search, study, comprehend and apply information gathered in current immunological journals/publications