Course Syllabus: TU M.Sc Microbiology First year: Microbial Structure, Physiology and Genetics

Microbial Structure, Physiology and Genetics

Course Title: Microbial Structure, Physiology and Genetics Full Marks: 100

Course No. MB 511 Pass Marks: 40

Nature of Course: Theory Year: I

Objectives

Upon the completion of the course students will have advanced knowledge on

• Bacterial and viral taxonomy
• The microbial genetics and application.
• Structure and physiology of bacteria, virus and fungi.
• Growth and recovery of bacteria and bacteriophage.

Microbial Structure and Physiology

Bacterial Classification

History, Fundamental and new approaches to bacterial taxonomy and nomenclature, Bacterial phylogeny, Characteristic of major families of bacteria (Gram positive, Gram negative, Mycobacteria, Actinomycetes, Rickettsia, Chlamydia, Mycoplama) 14 hrs

Molecular Structure and Composition of Bacterial Cell

Structure, physiology and functions of bacterial Cell wall, Cell membrane, Capsule, Spore, Flagella, Pili, Ribosome and other cellular structures 10 hrs

Bacterial Growth

Growth in individual cell, Batch and Continuous growth, Kinetics of bacterial growth, growth curve, Synchronization Procedures, Measurement of bacterial growth 6 hrs

Bacterial Metabolism

Transport mechanism of nutrients, Respiration and fermentation, Major energy yielding pathways and their significance, Electron transport chain, Oxidative and substrate level phosphorylation, Different types of fermentative pathways 12 hrs

Fungi: Structure and Physiology

Classification, Morphological and growth characteristics, Reproduction and life cycle of Yeast and Mold 8 hrs

Viruses: Structure, Classification and Replication

Structure of viruses, Classification schemes of bacteriophages and viruses, Replication, Enumeration, Culture and recovery of Viruses 10 hrs

Microbial Genetics

Overview

Molecular Structure and Function of DNA and RNA of Prokaryotic and Eukaryotic Cells 2 hrs

DNA Transfer in Procaryote

Types and Functions of Plasmids, Recombination (Homologous and Non-homologous), Transformation, Transduction (Generalized and Specialized), Conjugations, Genetic mapping 8 hrs

DNA Replication

Molecular Mechanism of DNA Replication in prokaryotic and eukaryotic cells, Enzymes involved in DNA replication: Topoisomerases, Helicases, DNA polymerases; Proofreading, post-replicational modification of DNA 8 hrs

Transcription

Role of RNA in transcription, Mechanism of RNA Synthesis, Initiation and Termination of Transcription, Post transcription modification of the RNA 5 hrs

Protein Biosynthesis

Role of RNA in protein biosynthesis, Translation of the genetic code, Steps involved in translation (Initiation, Elongation and Termination) 8 hrs

Regulation of the Gene Expression

Mechanism of Lac-operon and trp Operon: Gene expression in Eucaryotic cells 6 hrs

Mutations

Types of Mutation, Mutagenic agents: Physical, Chemical and Biological, Detection of Mutants 8 hrs

Recombinant DNA Technology

Principle, procedures and mechanism of gene cloning, Formation of the Recombinant DNA, Cloning vectors, Expression vectors, Detection of the recombinant DNA, Cloning of the eucaryotic genes in bacteria 8 hrs

Molecular Techniques

Extraction and purification of plasmid and chromosomal DNA and RNA, Principle, procedures and applications of PCR based techniques and blotting techniques in microbiology: Plasmid Profiling, PCR, Real time PCR, RFLP, DNA Finger printing, Western blotting, Southern blotting and Northern Blotting, PFGE, Gene Sequencing

7 hrs

Textbooks

1. Madigan MT, Martinko JM and Parker J. Brock's Biology of Microorganisms, 10^th Edition, Prentice-Hall International (2004).
2. Prescott LM, Haley JP and Klein DA. Microbiology, 7^th Edition (International Edition) McGraw Hill (2005).
3. Lewin B. Genes IX, Oxford University Press and Cell Press (2007).
4. Sambrook J and Russell DW. Molecular Cloning: A laboratory Manual, (Vol I, II & III) 3^rd Edition, Cold Spring Harbor Laboratory Press (2001).
5. Bergey's Manual of Systematic Bacteriology, Volume 1 (2001), Volume 2 (2005), Volume 3 (2009), Volume 4 (2009), Volume 5 (2009)
   
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