Friday, March 2, 2012

Course Syllabus: TU M.Sc Microbiology First year:Microbial Biotechnology and Pharmaceutical Microbiology


Microbial Biotechnology and Pharmaceutical Microbiology
Course Title: Microbial Biotechnology and Pharmaceutical Microbiology Full Marks: 100
Course No.: MB 514 Pass Marks: 40
Nature of course: Theory Year: I
Objectives
Upon the completion of the course students will have advanced knowledge on
  • Scope and application of Microbial Biotechnology
  • Production, purification, and product recovery of industrial products of microbial origin
  • Applications of Pharmaceutical Microbiology and quality control systems applied in pharmaceutical industry

Microbial Biotechnology
Overview: History, Scope, Areas, Application, Risk and Hazards of Biotechnology;
Gene Transfer Techniques: Vector Gene transfer (based on Ti and Ri Plasmid, intermediate and helper plasmid, binary vector, virus as vector), Gene transfer techniques using Agrobacterium; Animal Cell culture Technology, Plant cell and Tissue Culture Technology 10 hrs

Fermentation Technology

Fermentation Processes and Culture improvement: Introduction to industrial fermentation and bioprocess technology, Microorganisms of industrial importance, search and selection of new culture of industrial microorganisms. Strain development and Improvement of Industrial microorganisms. Contamination control mechanisms in the Fermentation Industry. Maintenance, quality control and purity of preserved stock culture. Management of culture collection; Bioengineering and Bioreactor Design: Basic concepts in Bioengineering; dimensions and units, equilibrium and rate, coefficients for mass and heat transfer, volumetric coefficient and mass balance. Bioreactor Design Fundamentals; introduction, volumetric oxygen transfer coefficient, aeration and agitation, scale up, impeller design, effect of stirring, sparging and other parameter, different type of bioreactors, operation and control of bioreactor system. Batch, fed-batch and continuous culture, solid state and submerged fermentation. Sterilization for fermentation. 15 hrs

Production, Purifications and Product recovery of Physiologically active substances by fermentation: Antibiotics (Penicillin, Streptomycin, Polypeptide antibiotics), enzyme inhibitors, Beta carotene, Riboflavin and vitamin B12, amino acids (glutamic acid, leucine, isoleucine, lysine, tryptophan, nucleic acids) Organic acids (Citric acid, Acetic acid, Lactic acid, Kojic acid), Enzymes (Amylase, Protease, Chitinase, Pectinase), Alcoholic beverages (Wine, Beer, Distilled liquor), Fermented Foods (Kinema, Soya sauce, NATO, Kimchi) and Single Cell Protein 15 hrs

Production and product recovery of: Human growth hormones, interferon, and insulin
2 hrs
Types, Applications and Production Process of Microbial bio-biofertilizers 2 hrs
Types, Applications and Production procedures of Bacterial, Fungal and Viral Biopesticides and Bioherbicides 2 hrs



Pharmaceutical Microbiology


Antibiotics
Sources, Types, Target and Mode of Action of Antibiotics, Antiviral drugs and Drug in combination 12hrs


Development of Drug Resistance in Bacteria
Types and Mechanisms of resistance 8hrs

Chemical Disinfectants, Antiseptics and Preservatives
Types, Mode of Action and Quality Evaluation 8hrs

Quality Evaluation of Pharmaceutical Products
Bioassays, Chemical assays and Immunoassay of Antibiotics, Quality Evaluation of disinfectants and preservatives 12hrs

Microbial Spoilage and Preservation of Pharmaceutical Products
Types of spoilage, factors affecting microbial spoilage, preservation of pharmaceutical products, quality assurance and management 10hrs
Sterilization Control and Sterility Testing of Pharmaceutical Products
Physical, chemical and biological indicators of sterilization, principles and methods of sterility testing 10hrs

Quality Control Systems
Conventional Systems, TQM, HACCP, Quality Assurance, Quality Auditing, GMP, GLP, ISO, Nepal Standard 14 hrs

Textbooks


  1. Smith JE. Biotechnology, 3rd Edition, Cambridge University Press (1996).
  2. Denyer SP, Hodges NA and Gorman SP (Eds) Hugo and Russell’s Pharmaceutical Microbiology, 7th Edition, Blackwell Science Ltd (2004).
  3. Crueger W and Crueger A. Biotechnology: A textbook of Industrial Microbiology. 2nd Edition, T. D. Brock's Editor. Sunderland Mass Sinauer Associates (1990).
  4. Cassida LE Jr, Industrial Microbiology, New Age Int. Publishers (reprint, 1996).
  5. Reed G (Ed) Prescott and Dunn's Industrial Microbiology, 4th Edition, Chapman & Hall (1981).

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