Microbial
Biotechnology and Pharmaceutical Microbiology
Course Title:
Microbial Biotechnology and Pharmaceutical Microbiology Full Marks:
100
Course No.: MB
514 Pass Marks: 40
Nature of course:
Theory Year: I
Objectives
Upon the completion of the course students will have
advanced knowledge on
- Scope and application of Microbial Biotechnology
- Production, purification, and product recovery of industrial products of microbial origin
- Applications of Pharmaceutical Microbiology and quality control systems applied in pharmaceutical industry
Microbial Biotechnology
Overview: History, Scope, Areas,
Application, Risk and Hazards of Biotechnology;
Gene Transfer Techniques: Vector Gene transfer
(based on Ti and Ri Plasmid, intermediate and helper plasmid, binary
vector, virus as vector), Gene transfer techniques using
Agrobacterium; Animal Cell culture Technology, Plant
cell and Tissue Culture Technology 10 hrs
Fermentation Technology
Fermentation Processes and Culture improvement:
Introduction to industrial fermentation and bioprocess technology,
Microorganisms of industrial importance, search and selection of new
culture of industrial microorganisms. Strain development and
Improvement of Industrial microorganisms. Contamination control
mechanisms in the Fermentation Industry. Maintenance, quality control
and purity of preserved stock culture. Management of culture
collection; Bioengineering and Bioreactor Design: Basic concepts in
Bioengineering; dimensions and units, equilibrium and rate,
coefficients for mass and heat transfer, volumetric coefficient and
mass balance. Bioreactor Design Fundamentals; introduction,
volumetric oxygen transfer coefficient, aeration and agitation, scale
up, impeller design, effect of stirring, sparging and other
parameter, different type of bioreactors, operation and control of
bioreactor system. Batch, fed-batch and continuous culture, solid
state and submerged fermentation. Sterilization for fermentation.
15 hrs
Production, Purifications
and Product
recovery of
Physiologically active
substances by
fermentation: Antibiotics (Penicillin,
Streptomycin, Polypeptide antibiotics), enzyme inhibitors, Beta
carotene, Riboflavin and vitamin B12, amino acids (glutamic acid,
leucine, isoleucine, lysine, tryptophan, nucleic acids) Organic acids
(Citric acid, Acetic acid, Lactic acid, Kojic acid), Enzymes
(Amylase, Protease, Chitinase, Pectinase), Alcoholic beverages (Wine,
Beer, Distilled liquor), Fermented Foods (Kinema, Soya sauce, NATO,
Kimchi) and Single Cell Protein 15 hrs
Production and
product recovery
of: Human growth hormones, interferon, and
insulin
2 hrs
Types, Applications and Production Process of
Microbial bio-biofertilizers 2 hrs
Types, Applications and Production procedures of
Bacterial, Fungal and Viral Biopesticides and Bioherbicides
2
hrs
Pharmaceutical Microbiology
Antibiotics
Sources, Types, Target and Mode of Action of
Antibiotics, Antiviral drugs and Drug in combination 12hrs
Development of Drug
Resistance in Bacteria
Types and Mechanisms of resistance 8hrs
Chemical Disinfectants,
Antiseptics and Preservatives
Types, Mode of Action and Quality Evaluation 8hrs
Quality Evaluation of Pharmaceutical Products
Bioassays, Chemical assays and Immunoassay of
Antibiotics, Quality Evaluation of disinfectants and
preservatives 12hrs
Microbial Spoilage and
Preservation of Pharmaceutical Products
Types of spoilage, factors affecting microbial
spoilage, preservation of pharmaceutical products, quality assurance
and management 10hrs
Sterilization Control and
Sterility Testing of Pharmaceutical Products
Physical, chemical and biological indicators of sterilization,
principles and methods of sterility testing 10hrs
Quality Control Systems
Conventional Systems, TQM, HACCP, Quality Assurance,
Quality Auditing, GMP, GLP, ISO, Nepal Standard 14
hrs
Textbooks
- Smith JE. Biotechnology, 3rd Edition, Cambridge University Press (1996).
- Denyer SP, Hodges NA and Gorman SP (Eds) Hugo and Russell’s Pharmaceutical Microbiology, 7th Edition, Blackwell Science Ltd (2004).
- Crueger W and Crueger A. Biotechnology: A textbook of Industrial Microbiology. 2nd Edition, T. D. Brock's Editor. Sunderland Mass Sinauer Associates (1990).
- Cassida LE Jr, Industrial Microbiology, New Age Int. Publishers (reprint, 1996).
- I.P./U.S.P./E.P.
More Syllabus of M.Sc First Year Microbiology (Tribhuvan University)
- Syllabus of M.Sc First year Immunology (TU)
- Syllabus of M.Sc First year Biochemistry-Instrumentation
- Syllabus of Microbial Biotechnology and Pharmaceutical Microbiology
- Syllabus of Microbial Structure, Physiology and Genetics
- Syllabus of Epidemiology, Research Methods and Biostatistics
- Syllabus of Practical Course on (MB 513+MB 514)
- Syllabus of Practical Course on (MB511+MB 512)
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