Course Syllabus: TU M.Sc Microbiology First year:Practical Course on (MB511+MB 512)

Practical I

Course Title: Practical Course on (MB511+MB 512) Full Marks: 50

Course No: MB516 Pass Marks: 20

Nature of course: Practical Year: I

Objectives

Upon the completion of the course, students will have

• practical skills on handling, isolation, identification and enumeration of bacteria, yeast and molds.
• advanced practical skills on molecular techniques to study microbial genetics.
• advanced practical skills on immunological and serological techniques.

I. Microbial Structure, Physiology and Genetics

Bacterial Morphology

• Microscopy and Staining

Microbial Physiology

• Measurement of growth of bacteria
• Enumeration techniques
• Biomass determination
• Determination of bacterial growth curve in broth medium
• Effect of environmental factors on bacterial growth
• Temperature, pH, UV radiation, nutrients, water activity, gaseous requirements
• Degradation experiments
• Cellulose, Starch, Gelatin, Casein, Tween 80
• To study inhibitory effect of lysozyme and detergents on bacterial cell wall
• Conventional biochemical testing for identification of enterobacteriaceae family
• Biochemical identification of unknown bacteria (Gram positive, gram negative)
• Antimicrobial Susceptibility Test

Yeast, Mold and Actinomyctes

• Isolation, enumeration and morphological identification of yeast and molds
• Growth of molds in different condition
• Isolation and characterization of Actinomycetes

Viruses

• Isolation and enumeration of bacteriophage
• One step growth curve of bacteriophage

Typing of bacteria

• Phage typing
• Antibiogram typing

II. Microbial Genetics

• Nucleus staining (Prokaryotic and Eukaryotic cells)
• Nucleic acid Extraction
• Extraction and purification of chromosomal and plasmid DNA from bacteria
• Extraction and purification of DNA from yeast cells

• Extraction and purification of RNA from Prokaryotic and Eukaryotic cells

• Determination of molecular weight of DNA (Agarose Gel Electrophoresis)

• DNA Transfer/Recombination/Expression

• Transformation experiment
• Conjugation experiment

• Mutation

• Generation, detection and isolation of mutants

• Molecular Techniques

• Plasmid Profiling

• PCR

• Blotting

   

   III. Immunology

• Handling of laboratory animals

• Animal inoculation Techniques using different routes

• Preparation of Antigen for animal inoculation

• Harvesting of antisera and purification of immunoglobulins
• Serological techniques
• Precipitation (Gel: Single and Ouchterlony double diffusion)
• Agglutination (Slide, Tube, Latex and Haemagglutination)
• Neutralization test
• ELISA
• Immunoelectrophoresis
• Immunoflourescence technique
• Immunochromatographic technique
• Complement Fixation test
• Hypersensitivity Reactions (Montoux test, Allergy test)
• Serotyping (E. coli, Salmonella, Vibrio)

More Syllabus of M.Sc First Year Microbiology (Tribhuvan University)

1. Syllabus of M.Sc First year Immunology (TU)
2. Syllabus of M.Sc First year Biochemistry-Instrumentation 
3. Syllabus of  Microbial Biotechnology and Pharmaceutical Microbiology
4. Syllabus of Microbial Structure, Physiology and Genetics 
5. Syllabus of Epidemiology, Research Methods and Biostatistics
6. Syllabus of Practical Course on (MB 513+MB 514) 
7. Syllabus of Practical Course on (MB511+MB 512)