Practical II
Course Title: Practical
Course on (MB 513+MB 514) Full Marks: 50
Course No. MB 517
PassMarks: 20
Nature of Course:
Practical Year: I
Objectives
Upon the completion of
the course, students will have
- practical skills on various biochemical techniques and instrumentation, required for conducting analysis, research.
- practical skills on production, purification and quality control of industrial products of microbial origin.
- practical skills on production, purification, evaluation and sterility testing of pharmaceutical products.
I. Microbial Biochemistry and Instrumentation
- Preparation of buffers, titration curves of amino acids (Glycine and Tyrosine).
- Techniques of filtration and dialysis
- Extraction of glycogen from liver
- Separation of amino acids by single and double ascending paper chromatography
- Separation of sugars by thin layer chromatography,
- Separation of various lipids by thin layer chromatography,
- Separation of amino acids and proteins by ion-exchange chromatography
- Analysis of serum proteins by Electrophoresis using cellulose acetate papers,
- Determination of molecular weight of proteins by SDS-PAGE,
- Purification proteins by Gel filtration,
- Application of colorimetry, spectrophotometry and fluorimetry,
- Protein estimation by Folin-Lowery methods
- Ammonium sulfate precipitation of serum proteins,
- Determination of iso-electrical points of protein,
- Quantitative determination of blood sugar level
- Quantitative determination of cholesterol in blood,
- Determination of enzyme activities: ALT, GOT, amylase
- Determination of enzyme kinetics km and Vmax
II. Microbial Biotechnology
- Student model fermenter design
- Isolation and identification of Rhizobium spp.
- Isolation and Identification of Bacillus thuriengiensis and purification of crystal protein.
- Production, purification and characterization of industrially important enzymes of microbial origin.
- Production of Wine and Sake.
- Production, optimization and purification of local alcoholic beverages
- Distillation of alcoholic beverages and estimation of alcohol percentage.
- Determination of methanol in distilled liquors.
- Production, separation, partial purification and characterization of Antimicrobial compounds from Bacillus spp. and Actinomycetes.
- Production and purification of organic acids using microorganisms.
- Field Visit to Industries
III. Pharmaceutical Microbiology
- Antibiotic Susceptibility Testing: Disc Diffusion methods
- Determination of minimum inhibitory concentration of antibiotics and other antimicrobial compounds by tube and agar dilution methods; E-Test
- Monitoring of sterilization
- Sterility and quality testing of Pharmaceutical Products
- Testing of efficacy of disinfectants
- Microbial limit test of raw materials used in pharmaceutical industries
- Microbial monitoring of environment of pharmaceutical industries.
More Syllabus of M.Sc First Year Microbiology (Tribhuvan University)
- Syllabus of M.Sc First year Immunology (TU)
- Syllabus of M.Sc First year Biochemistry-Instrumentation
- Syllabus of Microbial Biotechnology and Pharmaceutical Microbiology
- Syllabus of Microbial Structure, Physiology and Genetics
- Syllabus of Epidemiology, Research Methods and Biostatistics
- Syllabus of Practical Course on (MB 513+MB 514)
- Syllabus of Practical Course on (MB511+MB 512)
No comments:
Post a Comment
If you like this post or have any issues please do leave me comment